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Reactive Oxygen Species (ROS) Assay Kit detects reactive oxygen species using fluorescent probe DCFH-DA. DCFH-DA itself has no fluorescence and can freely pass through the cell membrane. After entering the cell, it is hydrolyzed by intracellular esterase into a non-fluorescent molecule. ROS in the cell oxidises this into 2’, 7’–dichlorofluorescein (DCF). DCF is highly fluorescent and is detected by fluorescence spectroscopy with excitation / emission at 485 nm / 535 nm.
Call No. | Packing | Price |
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CA1410 | 100T | 22000 |