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Acid Phosphatase (ACP) Assay Kit

In acid condition, ACP catalyse phosphomonoester to inorganic phosphate, which is found in lysozyme of macrophages. ACP can be used in auxiliary diagnosis of prostate cancer. In acid condition, ACP catalysis hydrolysis disodium phenyl phosphate to phenol, and the phenol reacts with 4-aminoantipyrine and potassium ferricyanide to form red quinone derivative, which can be detected by its absorbance at 510 nm. ACP activity can be calculated by measuring the absorbance increase rate at 510nm.

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